fmol / µg membrane protein (%CV)a,b Donor (Gender, Age, Ethnicity) OATP1B1 OATP1B3 OATP2B1 Male, 66, Unknown 14.9 ± 0.4 (7.8) 6.6 ± 0.2 (7.9) 3.6 ± 0.2 (13.7) Male, 45, Asian 13.7 ± 0.4 (9.0) 11.8 ± 0.2 (4.9) 4.6 ± 0.2 (12.1) Male, 50, Asian 3.1 ± 0.1 (12.0) 2.8 ± 0.1 (12.0) 1.2 ± 0.1 (12.0) Female, 65, Unknownc 10.6 ± 0.3 (4.6) 2.4 ± 0.1 (3.8) 2.0 ± 0.1 (5.3) aMembrane fractions were initially denatured in 25 mM ABC containing 10% DOC prior to alkylation, dilution, and digestion. bData represent the mean ± S.E.M. and coefficient of variation (CV%) for three transitions measured for each peptide by LC-MS/MS for 44 hr digestions processed in triplicate. cFinal quantification values for the fourth liver tissue are representative of the mean of three transitions measured for each peptide for a single sample.

Table 2: Quantification of OATP1B1 (surrogate-1), OATP1B3 (surrogate-2), and OATP2B1 (surrogate-1) peptides in human liver membrane fractions.