| Sl No. |
Target Gene |
Primer Sequence |
Annealing Temperature |
| 1 |
Pyruvate kinase |
F:SCAGYTGTTTGAGGAGCTAC |
52°C |
| R:CCTGTCACCACAATCACCAG |
| 2 |
Creatine Kinase |
F:TCACCCTGCCTCCTCACAA |
42°C |
| R:TGCCCTTGAACTCACCATCC |
| 3 |
Beta actin |
F:AGAGAGAAATTGTCCGTGACATC |
60°C |
| R:CTCCGATCCAGACAGAGTATTTG |
| 4 |
Phosphorylase kinase |
F:GACAGGGACATGGACTGACA |
50°C |
| R:AACCACTTCCAACAGGGAGC |
| 5 |
Adenylate Kinase |
F:CATGATCGCCAAAGCTGACG |
45°C |
| R:CACTGGCAGCTCAGAGTCAA |
| 6 |
Enolase |
F:ATGCTGGCGATCAAAATCATA |
47°C |
| R:CTGCTGTTTCTCAATGGCTCT |
| 7 |
Phosphohistidine phosphatase |
F:CTTCGCTGTTGTGTTTGGGG |
47°C |
| R:ACTCCTCCAGCTCTCTCCAG |
| 8 |
PDZ and LIM |
F:GACAGAAATGCCACAGAACG |
47°C |
| R:TCATTTGCGTGAGGTCTGAG |
| 9 |
CapZB |
F:CGCTGGCGGATAAAACGAAG |
50°C |
| R:CGGGTGGAGGGTGAAACTAC |
| 10 |
Aspartate aminotransferase |
F:CATGCCTGTGCCCATAACC |
46°C |
| R:CACCAGGTCAGCGATCTCTTT |
| 11 |
Glyceraldehyde 3 phosphate dehydrogenase |
F:CTGGTGACCCGTGCTGCTT |
42°C |
| R:TTTGCCGCCTTCTGCCTTA |
| 12 |
Apolipoprotein |
F:CCTTCTCCATCTGCTCCCTATAA |
45°C |
| R:CTCCACGGCTACTTTCAGAACG |
| 13 |
Fructose 1,6 bisphosphate |
F:AAFMGKACCATYCAGACMGC |
50°C |
| R:CAGCYTGRTGRCAGATSACC |
| 14 |
Lactate Dehydrogenase |
F:AGTACAGCCCCAACTGCATC |
51°C |
| R:CCACATTCACACCACTCCAC |
| 15 |
Phosphoglycerate kinase |
F:AGGGGCCAAGGTCAAAGATA |
48°C |
| R:CCCTGTTGTTGCCTTCTCAT |
| 16 |
Transferrin * |
F: GGACTACCAGCTGTTGTGCAT |
64 °C |
| R: GCCACCATCGACTGCAAT |
| 17 |
hsp47 |
F: CACTGGGATGAGAAGTTCCA |
59°C |
| R: AAGGAAAATGAAGGGATGGTC |
| 19 |
hsp60 |
F: AAAGATGGGGTCACAGTTGC |
54°C |
| R: TGTTGAGGACCAGAGTGCTG |
| 17 |
hsp70 |
F: GCATGGTGAACCACTTTGTG |
53°C |
| R: CTCTGCCGTTGAGAAATCC |
| 18 |
hsc71 |
F: TGAAGCACTGGCCTTTTAAT |
60°C |
| R: CCAAGCAGTAGATTTGACCTC |
| 20 |
hsp90 |
F:GGAAATCTTCCTCCGAGAGC |
52°C |
| R: CCGAATTGACCGATCATAGA |
| 21 |
18s RNA |
F:GGAGGTTCGAAGACGATCAG |
62°C |
| R:AACCAGACAAATCGCTCCAC |
Source: [28]