![]() |
Figure 1: Representative gel lanes for quantitative mass spectrometric analysis of MCC and carcinoid tumor of the lung from formalin-fixed paraffin-embedded (FFPE) tissues. Proteins were extracted from 10 MCC FFPE tissues and 5 carcinoid tumors of the lung FFPE tissues. The samples were equally loaded and resolved by sodium dodecyl sulfate polyacrylamid gel electrophoresis (SDS-PAGE)/Coomassie. Each gel lane was sliced into 23 bands, subjected to trypsin digestion and peptides were analyzed by liquid chromatography-mass spectrometry (LC-MS)/MS with a Thermo LTQ-XL mass spectrometer. Proteins were identified by a Mascot database search (95% confidence threshold). One gel lane from each group is shown. |