Figure 5: mTOR inhibition suppresses LDHB and hnRNPF expression in both MCC-2 and MCC-3 cells. (A) Suppressed LDHB and hnRNPF mRNA expression in MCC-2 and MCC-3 cells. MCC-2 and MCC-3 cells were treated with DMSO, Ku-0063794 (5μM) and PP242 (2.5μM) for 24 hours, respectively. cDNAs were extracted and expression of hnRNPF and LDHB was analyzied by qPCR. Triplicate runs of each sample were normalized to MRPS2 mRNA to determine relative expression (means ± SEM), (*P < 0.05, ***P< 0.001). (B) Suppressed mTOR pathway, LDHB and hnRNPF protein expressions in MCC-2 and MCC-3 cells by immunoblottings. MCC-2 and MCC-3 cells were treated with DMSO, Ku-0063794 (5μM) and PP242 (2.5μM) for 24 hours, respectively. Lysates were prepared and subjected to immunoblotting analysis with indicated antibodies. β-actinwas served as proper loading control.