Figure 1: A description of the peak detection principle, which is used in the BIOSPEAN:
a) A step-by-step scanning is performed around all m/z positions, which examines local intensity values. For a given position x, the surroundings are checked for the highest, lowest and average intensity.
b) Minimum, mean and maximum noise levels are determined. Signals with intensity values exceeding noise are considered peaks; green line – minimum noise (set arbitrarily at zero); green dashed line – average noise (1 unit); green dotted line – maximum noise (2 units); blue line – a threshold line for peak detection (2.5 units) taking possible noise extremes into consideration.
c) The peak positions found and their surroundings are finally marked and assessed by points.