Figure 2: Manual annotation of gene 1 predicted with Augustus software and showing tight linkage to soybean rust disease marker Satt460 (SSR repeat) with an AT base insertion located within the intron (non coding region). Two exons (coding regions) in the predicted gene 1 is displayed with different sizes and interspersed with introns. The splice sites are not part of the coding region but show the probable regions of DNA slicing of mRNA post-translation modifications during protein synthesis.