a) Statistics of the top 15 experimental methods that detected proteins is displayed. For every method, a table containing total number of annotations and unique proteins is shown below the histogram. MS had the most number of protein annotations (752,211) with 18,985 unique proteins (93% of the human proteome). Note that the units on y-axis (number of annotations) are altered to accommodate high numbers (MS and IHC). RC – reconstituted complex; coip – co- immunoprecipitation; Fusion proteins – fusion proteins for subcellular localization. b) Statistics of cancer proteome is displayed. Unique number of proteins identified in various cancer tissues and cell lines is plotted as a bar graph. All represents the total cancer proteome (i.e., proteins identified in all cancerous tissues and cell lines). Ovarian cancer had 10,643 unique proteins expressed at various time points followed by colorectal cancer with 9,031 unique protein identifications.

Figure 3: Experimental methods and cancer proteome based distribution of the human proteome.