Figure 5: Temporal expression of myogenin during embryonic development with whole-mount in situ hybridization (upper panel) and Real time RT-PCR (Lower panel) as described in Materials and methods. The typical embryonic development stages are: 1, fertilized egg (2 min pf), 2, Blastula (30 min pf), 3, Gastrula (3 hpf), 4, 3 somite stage (12 hpf), 5, 7 somite stage (18 hpf), 6, 12 somite stage (24 hpf), 7, 24 somite stage (30 hpf), 8, 48 somite stage (36 hpf), 9, Hatching stage (48 hpf) and 10, free swimming fry (50 hpf). Embryos are viewed laterally, anterior to the up, and all are at the same magnification (×100). Arrow indicates somite formation in anterior to caudal wave fashion. Scale bar =100 μm.