| Primer name |
Sequence(5’-3’) |
Information |
| MG1 |
ATGGAGCTTTTCGAGACC |
The exon1 and intron1 amplication |
| MG2 |
TATTTGGGAATGTCCACAGG |
The exon1 and intron1 amplication |
| MG3 |
GGCCTTAAAGCCCTAGAG |
The 5’-flanking region amplication |
| MG4 |
GGAAGAAGTAAGGGTTGGT |
The 5’-flanking region amplication |
| MG5 |
CTGTGGACATTCCCAAGTAG |
The 3’- flanking region amplication |
| MG6 |
AGCATTAGAAACACGCACAAA |
The 3’- flanking region amplication |
| MG7 |
CTATTTGGGAATGTCCACAGG |
The myogenin cDNA amplication |
| MG8 |
ACCAACCCTTACTTCTTCCCT |
Whole-mount in situ hybridization |
| MG9 |
TCCCTGCTGTCCCGTCTC |
Whole-mount in situ hybridization |
| MG10 |
GCACCCTGATGAACCCAA |
Quantitative real-time PCR assay |
| MG11 |
CTCGCTTGATGACGACACTC |
Quantitative real-time PCR assay |
| Actin1 |
CCTTCTTGGGTATGGAGTC |
Quantitative real-time PCR assay |
| Actin2 |
GTCAGCAATGCCAGGGTA |
Quantitative real-time PCR assay |
