Figure 2: Chromatin immunoprecipitation (ChIP) analysis of TFCP2 protein (CP2-IP) demonstrating specific binding of TFCP2 to promoter regions of two selected M8 motif-bearing genes (RBP-j-kappa and PurA) in vivo. One selected region, localizing 1.8 downstream to the M8 motif of the RBP-j-kappa gene and a region, localizing 1.3kb downstream to the M8 motif of the PurA gene served as controls for background DNA binding. Rabbit pre-immune serum (Control-IgG) was used as the specificity control for the anti TFCP2 antibody. The experiments were conducted in triplicate (error bars show SD).