Figure 2: Human nephrin antibody. A) A peptide corresponding to N-terminal amino acids 1039-1056 of human nephrin (highlighted in gray) was employed to make a new antibody. Input parameters for AbDesigner: Input Type = Gene Symbol-Human, Input = NPHS1, Peptide Length = 18, Epitope Length = 7. B) Alignment of the human peptide to mouse and rat nephrin. C) An immunoblot of human kidney cortex, mesangial cell, and liver samples probed with the K3 rabbit polyclonal antibody.