Figure 3: Human podocin antibody. A) A carboxyl-terminal peptide (amino acids 366-383) of human podocin (highlighted in gray) was utilized for the production of a new antibody. Input parameters for AbDesigner: Input Type = Gene Symbol-Human, Input = NPHS2, Peptide Length = 18, Epitope Length = 7. B) Immunofluorescence staining of mouse frozen kidney and liver sections with the K2 rabbit polyclonal antibody, different molar ratios of the antibody and blocking peptide were used. Arrowheads indicate glomeruli with the distinctive ribbon-like pattern corresponding to podocyte staining.