Figure 1: 2D images and system reproducibility for the Auto2D. A. Cy5 images of HuO9 osteosarcoma cells obtained using the Auto2D. The gel size was 6.5 × 3.9 cm, and the separation area was 3.0 × 3.9 cm. Note that the protein spots were well focused and separated across the entire area. B. A typical scattergram of protein spots in gels 1 and 2. Among 521 protein spots observed, the intensity of 414 was scattered within a 2-fold range of differences. The correlation coefficient was 0.66 for all 521 protein spots, and 0.87 for the 414 protein spots.