Figure 2

Figure 2: Free folding energies in different codon residues. Free folding energies (FFE) were determined in phase-selected sub-sequences of 81 genes (random selection from human sequence database). The original nucleic acids contained intact three-letter codons (1^st+2^nd+3^rd). Sub-sequences were constructed by periodic removal of one letter from the codon and maintaining the other two (1^st+2^nd, 1^st+3^rd, 2^nd+3^rd), or removing two letters and maintaining only one (1^st, 2^nd, 3^rd). Distinctions were made between exons and the preceding (-1) and following (+1) sequences (introns). The dG values were determined using mfold and the FFE was calculated. Each bar represents the mean ± SEM, n = 81 [9].