Accession No. Log2 ratio p-valuea Peptideb Protein Decrease of reduced form (-SH) alkylated with MPB CALVIN CYCLE ATCG00490 -0.33 5.0 × 10-11 422VALEACMPBVQAR Ribulose bisphosphate carboxylase large chain (RBCL) Increase of oxidized form (-S-S-) alkylated with BIAM CALVIN CYCLE AT1G67090, 0.43 2.9 × 10-2 91,91,91,91NKWIPCBIAMVEFELEHGFVYR RuBisCO small subunit AT5G38430, 0.26 9.2 × 10-5 141,141,141,141EVEECBIAMKK 1A/1B/2B/3B AT5G38420, 0.16 4.4 × 10-4 127,127,127,127LPLFGCBIAMTDSAQVLK (RBCS1A/1B/2B/3B) AT5G38410         AT2G36460, AT3G52930 0.42 7.3 × 10-4 197,197CBIAMAAVTER Fructose-bisphosphate aldolase 6/8 (FBA6/8) AT1G32060 0.26 1.4 × 10-3 292KLTCBIAMSYPGIK Phosphoribulokinase (PRK) AT2G39730 0.22 1.4 × 10-6 172SFQCBIAMELVMAK RuBisCO activase (RCA) AT2G21170 0.20 3.1 × 10-2 199TFDVCBIAMFAQLK Triosephosphate isomerase (TIM) TRANSLATION-RELATED PROTEINS AT1G14320, AT1G26910, AT1G66580 0.87 4.8 × 10-2 122,122,122ALGTCBIAMAR Ribosomal protein L10 A/B/C (RPL10A/B/C) AT3G48930; AT4G30800; AT5G23740 0.48 3.8 × 10-2 59;59;59CBIAMPFTGTVSIR Embryo defective 1080 (EMB1080); Nucleic acid-binding, OB-foldlike protein; Ribosomal protein S11-beta (RPS11-BETA) AT4G20360; AT4G02930 0.45 2.1 × 10-3 143;131HYAHVDCBIAMPGHADYVK RAB GTPase homolog E1B (RABE1b); GTP binding Elongation factor Tu family protein AT3G11940, AT2G37270 0.23 4.6 × 10-2 67,67AQCBIAMPIVER Ribosomal protein 5A/B (RPS5A/B) PHOTOSYNTHESIS AT5G66570, AT3G50820 0.34 1.5 × 10-3 135,134KFCBIAMFEPTSFTVK Photosystem II subunit O-1/2 (PSBO1/2) AT4G02770, AT1G03130 0.32 1.6 × 10-5 132,128KEQCBIAMLALGTR Photosystem I subunit D-1/2 (PSAD-1/2) LIPID TRANSPORT AT2G10940 0.25 0.23 2.7 × 10-4 4.2 × 10-2 215LGACBIAMVDLLGGLVK 206ATCBIAMPIDTLK Bifunctional inhibitor/lipidtransfer protein/seed storage 2S albumin superfamily protein FERREDOXIN/THIOREDOXIN SYSTEM AT1G10960, AT1G60950 0.44 4.4 × 10-8 93,93AGSCBIAMSSCBIAMAGK Ferredoxin 1/2 (FD1/2) aGiven by two-tailed Student’s t-test. All peptides listed were selected using two-tailed Student’s t-test with Benjamini-Hochberg multiple hypothesis testing correction (FDR ≤ 5 %). bThe cysteines (C) labeled by MPB and BIAM are marked by superscript MPB and BIAM, respectively.

Table 1: Oxidation of cysteine residues induced by 10-minute H2O2 treatment.