Figure 3: Elevated pressure improves protein extraction from model FFPE tissue surrogates. FFPE tissue surrogates were heated in 50 mM Tris, pH 8+2% SDS at either elevated pressure (40,000 psi) or atmospheric pressure. The electrophoretic mobility of the tissue surrogate extracts were compared to the native, unfixed tissue surrogate mixture by 1D-PAGE. Lane M: molecular weight marker; lane 1: native, unfixed tissue surrogate mixture; lane 2: FFPE tissue surrogate with 2.5% myoglobin after retrieval at 40,000 psi; lane 3: FFPE tissue surrogate with 5% myoglobin after retrieval at 40,000 psi,; lane 4: FFPE tissue surrogate with 5% myoglobin after retrieval at atmospheric pressure (14.7 psi).