| Sample type |
Buffer pH |
Extraction Pressure |
% False protein IDs* |
| Native, unfixed protein mixture |
N/A |
N/A |
3.3 ± 0.6 |
| Tissue surrogate |
4 |
14.7 psi ** |
42 ± 4.0 |
| Tissue surrogate |
4 |
40,000 psi |
7.8 ± 1.5 |
| Tissue surrogate |
8 |
14.7 psi |
100 |
| Tissue surrogate |
8 |
40,000 psi |
5.7 ± 1.1 |
*Determined as percentage of proteins incorrectly identified for spectra for 2
technical replicates. **Atmospheric pressure. N/A=not applicable. |
