Spot no.a Accession no.b Symbol Identified protein P value Fold pIc MWc Protein Peptide Peptide sequence
difference (obs) (obs)(Da) scored matches coverage (%)
790 Q07065 CKAP4 Cytoskeleton-associated protein 4  2.95E-03 6.502 5.63 66097 1007 21 38
1084 B4E102 IF4A1 Eukaryotic initiation factor 4A-I  1.86E-03 7.837 5.32 46353 68 1 2.5
1313 Q92890 UFD1 Ubiquitin fusion degradation protein 1 homolog  1.55E-04 8.6 6.27 34763 59 1 4.9
1315 Q6IBS0 TWF2 Twinfilin-2  1.55E-04 9.508 6.37 39751 593 13 36.1
1345 O15372 EIF3H Eukaryotic translation initiation factor 3 subunit H  1.55E-04 7.07 6.09 40076 102 2 5.4
1369 P07355 ANXA2 Annexin A2  6.22E-04 6.512 7.57 38808 316 6 18.3
1393 P07910 HNRPC Heterogeneous nuclear ribonucleoproteins C1/C2  1.55E-04 6.699 4.95 33707 192 5 15.4
1434 Q14966 ZN638 Zinc finger protein 638  1.55E-04 6.594 6.02 221914 48 1 1.2
1478 O15287 FANCG Fanconi anemia group G protein  1.55E-04 6.917 5.32 69423 45 1 2.7
1505 G3V2C9 GBLP Guanine nucleotide-binding protein subunit beta-2-like 1  3.11E-04 6.878 7.6 35511 508 8 30
1520 P40926 MDHM Malate dehydrogenase, mitochondrial  2.95E-03 8.009 8.92 35937 48 1 3.3
1522 P04818 TYSY Thymidylate synthase  6.22E-04 9.901 6.51 35978 440 9 28.1
1541 Q9HC38 GLOD4 Glyoxalase domain-containing protein 4  6.22E-04 6.622 5.4 35170 51 1 4.2
1585 P00491 PNPH Purine nucleoside phosphorylase  1.55E-04 8.418 6.45 32325 784 20 55.4
1619 Q9H0J4 QRIC2 Glutamine-rich protein 2  1.09E-03 6.97 6.25 181228 40 1 0.5
1706 P49736 MCM2 DNA replication licensing factor MCM2  3.11E-04 6.343 5.34 102516 42 1 2.7
1746 Q9Y2X7 GIT1 ARF GTPase-activating protein GIT1  6.22E-04 8.352 6.33 85030 42 1 2.1
1760 Q8NBJ7 SUMF2 Sulfatase-modifying factor 2  3.11E-04 9.799 7.79 33950 83 2 8.6
1792 P25940 CO5A3 Collagen alpha-3(V) chain  1.55E-04 6.524 6.37 172631 40 1 0.9
1794 Q6ZN55 ZN574 Zinc finger protein 574  1.55E-04 6.139 8.44 101175 35 1 2.1
1810 O00299 CLIC1 Chloride intracellular channel protein 1  1.55E-04 11.274 5.09 27248 296 4 20.7
1811 Q9HCI6 CLIC1 Chloride intracellular channel protein 1  1.55E-04 7.218 5.09 27248 276 4 20.7
1826 Q9UL46 PSME2 Proteasome activator complex subunit 2  1.55E-04 7.675 5.44 27515 280 5 23
1830 O00299 CLIC1 Chloride intracellular channel protein 1  1.55E-04 6.377 5.09 27248 227 3 17.4
1846 Q9HCI6 K1529 Uncharacterized protein KIAA1529  1.55E-04 6.745 5.74 192404 48 2 1.2
1869 Q9HCI7 K1529 Uncharacterized protein KIAA1529  6.22E-04 7.416 5.74 192404 46 4 1.2
1873 P62318 SMD3 Small nuclear ribonucleoprotein Sm D3  6.22E-04 6.372 10.33 14021 35 1 8.7
2180 P23528 COF1 Cofilin-1  1.09E-03 6.025 8.22 18719 293 4 37.3
2181 Q9UQ35 SRRM2 Serine/arginine repetitive matrix protein 2  1.55E-04 7.99 12.05 300179 36 1 1.1
2242 P18085 ARF4 ADP-ribosylation factor 4  1.55E-04 6.686 6.59 20612 168 3 15.6
2252 P02792 FRIL Ferritin light chain  6.22E-04 11.458 5.51 20064 125 2 17.1
2397 C9K028 NDKA Nucleoside diphosphate kinase A  6.22E-04 6.401 5.83 17309 447 13 64.5
2504 O15511 ARPC5 Actin-related protein 2/3 complex subunit 5  1.55E-04 6.712 5.47 16367 293 7 29.1
3334 P50579 AMPM2 Methionine aminopeptidase 2  6.99E-03 9.594 5.57 53713 36 1 3.6
3635 Q07065 CKAP4 Cytoskeleton-associated protein 4  1.55E-04 6.066 5.63 66097 1209 18 38.7
3636 P30101 PDIA3 Protein disulfide-isomerase A3  1.55E-04 6.026 5.98 57146 1312 25 46.1
3731 P0C881 R10B1 Radial spoke head 10 homolog B  1.09E-03 8.385 7.16 101255 35 1 2
3754 Q8IVM7 CM029 Uncharacterized protein C13orf29  1.09E-03 6.89 9.29 18425 39 1 11
3834 P08670 VIME Vimentin  6.22E-04 11.877 5.06 53676 1794 41 60.3
3838 E9PBJ4 TBB5 Tubulin beta chain  6.22E-04 9.029 4.78 50095 301 8 13.7
3927 Q9VKI9 PO2F3 POU domain, class 2, transcription factor 3  2.95E-03 7.14 8.81 47764 36 1 3.9
3928 E9PBJ4 TBB5 Tubulin beta chain  6.22E-04 9.912 4.78 50095 310 7 15.3
3931 P61158 ARP3 Actin-related protein 3  3.11E-04 6.479 5.61 47797 618 12 25.6
3934 O00148 DDX39 ATP-dependent RNA helicase DDX39  1.09E-03 9.134 5.46 49611 533 10 25.1
3942 Q9Y265 RUVB1 RuvB-like 1  1.09E-03 6.908 6.02 50538 175 4 8.6
3946 E7EQ64 TRY1 Trypsin-1  6.22E-04 6.405 6.08 27111 46 1 4
3964 Q9Y265 RUVB1 RuvB-like 1  1.09E-03 6.829 6.02 50538 984 17 37.3
4026 P08670 VIME Vimentin  2.95E-03 9.717 5.06 53676 167 3 7.9
4041 O95996 APC2 Adenomatous polyposis coli protein 2  4.66E-03 6.069 9.08 245966 36 1 0.6
4089 P52597 HNRPF Heterogeneous nuclear ribonucleoprotein F  1.55E-04 10.688 5.38 45985 146 2 8
4091 Q8IYK8 REM2 GTP-binding protein REM 2  1.55E-04 6.024 9.19 36170 38 1 8.5
4352 B1AK85 K1529 Uncharacterized protein KIAA1529  1.55E-04 6.957 5.74 192404 44 3 1.2
4399 Q9NY93 DDX56 Probable ATP-dependent RNA helicase DDX56  1.55E-04 6.235 9.34 62007 37 1 3.8
4408 Q9UJ70 NAGK N-acetyl-D-glucosamine kinase  1.55E-04 9.736 5.81 37694 216 4 12.8
4409 Q9UJ70 NAGK N-acetyl-D-glucosamine kinase  1.55E-04 6.083 5.81 37694 513 8 28.5
4521 Q6P1NO C2D1A Coiled-coil and C2 domain-containing protein 1A  6.22E-04 7.158 8.22 104397 51 1 1.6
4524 Q6DN90 IQEC1 IQ motif and SEC7 domain-containing protein 1  1.09E-03 6 6.49 109103 34 1 1
4526 P00338 LDHA L-lactate dehydrogenase A chain  6.99E-03 6.88 8.44 36950 247 4 12
4532 A6NHQ2 FBLL1 rRNA/tRNA 2~-O-methyltransferase fibrillarin-like protein 1  1.09E-03 8.67 10.33 34711 41 1 3.6
4533 Q14315 FLNC Filamin-C  1.09E-03 10.24 5.68 293344 39 1 0.4
4584 P47756 CAPZB F-actin-capping protein subunit beta  1.09E-03 6.927 5.36 31616 518 11 28.2
4586 P47756 CAPZB F-actin-capping protein subunit beta  1.55E-04 6.478 5.36 31616 431 9 21.7
4587 Q53EZ4 CEP55 Centrosomal protein of 55 kDa  1.55E-04 9.03 6.52 54433 38 1 2.6
4652 Q969P0 IGSF8 Immunoglobulin superfamily member 8  1.55E-04 13.696 8.23 65621 35 1 1.6
4661 Q8WZ26 YS006 Putative uncharacterized protein PP6455  1.55E-04 6.794 8.26 15165 35 1 6.7
4680 Q8NBJ7 SUMF2 Sulfatase-modifying factor 2  1.55E-04 7.584 7.79 33950 119 2 8.6
4681 P40261 NNMT Nicotinamide N-methyltransferase  1.55E-04 12.373 5.56 30011 239 4 17.4
4682 P40261 NNMT Nicotinamide N-methyltransferase  1.55E-04 8.947 5.56 30011 104 2 7.6
4714 Q14694 UBP10 Ubiquitin carboxyl-terminal hydrolase 10  1.55E-04 7.147 5.19 87707 35 1 1.9
4728 O00299 CLIC1 Chloride intracellular channel protein 1  1.55E-04 11.665 5.09 27248 320 5 24.1
4729 O00299 CLIC1 Chloride intracellular channel protein 1  1.55E-04 10.708 5.09 27248 176 3 17.4
4742 O00299 CLIC1 Chloride intracellular channel protein 1  3.11E-04 6.383 5.09 27248 60 1 5
4754 P08670 VIME Vimentin  2.95E-03 6.636 5.06 53676 212 4 10.5
5076 C9J035 B3A2 Anion exchange protein 2  1.55E-04 6.587 5.9 137493 41 1 1
5097 P84085 ARF5 ADP-ribosylation factor 5  4.66E-03 7.633 6.3 20631 278 5 34.4
5193 Q16853 AOC3 Membrane primary amine oxidase  3.11E-04 6.428 6.05 85138 37 1 2.5
5197 P23284 PPIB Peptidyl-prolyl cis-trans isomerase B  3.11E-04 8.197 9.42 23785 427 11 34.7
5198 P23284 PPIB Peptidyl-prolyl cis-trans isomerase B  1.55E-04 6.554 9.42 23785 202 5 23.6
5578 P14314 GLU2B Glucosidase 2 subunit beta  6.22E-04 6.496 4.33 60357 205 3 8.1
5668 P27797 CALR Calreticulin  3.11E-04 6.218 4.29 48283 213 5 8.6
5709 P30101 PDIA3 Protein disulfide-isomerase A3  3.11E-04 9.99 5.98 57146 565 12 22.8
5710 P30101 PDIA3 Protein disulfide-isomerase A3  6.22E-04 8.527 5.98 57146 696 12 26.1
5870 O60583 CCNT2 Cyclin-T2  6.22E-04 7.96 9.04 81492 39 1 1.6
5872 P08670 VIME Vimentin  6.22E-04 7.763 5.06 53676 952 22 37.1
5928 Q9H3Z4 DNJC5 DnaJ homolog subfamily C member 5  6.22E-04 6.198 4.93 22933 43 1 10.1
5952 Q07065 IF4A1 Eukaryotic initiation factor 4A-I  6.22E-04 15.257 5.32 46353 55 1 2.5
5953 Q07065 IF4A1 Eukaryotic initiation factor 4A-I  3.11E-04 8.525 5.32 46353 487 10 23.4
6062 Q15019 SEPT Septin-2  6.22E-04 6.664 6.15 41689 142 3 8.6
6126 Q14847 LASP1 LIM and SH3 domain protein 1  1.55E-04 7.205 6.61 30097 189 3 15.3
6127 B4DHY1 HNRH3 Heterogeneous nuclear ribonucleoprotein H3  1.55E-04 6.059 6.37 36960 61 1 3.5
6136 Q5TB53 TM9S3 Transmembrane 9 superfamily member 3  1.09E-03 8.325 6.83 68584 36 1 2
aSpot numbers refer to those in Figure 1B.
bAccession numbers of proteins were derived from Swiss-Plot and NBCI nonredundant data bases.
cObserved isoelectric point and molecular weight calculated according to location on the 2D gel.
dMascot score for the identified proteins based on the peptide ions score (p < 0.05) (http://www.matrixscience.com).
Table 2: A list of identified proteins with differential expression between tumor and non-tumor tissues in ES patients.