To determine the LD10/LD 50 and estimate the minimal cytotoxic concentration that would be used to establish a chronic cell exposure condition. The exposure of HaCaT cells to arsenic had LD10 and LD50 values of 1ppm and 10ppm respectively. The LD10 dose result obtained from the initial cytoxicity assay was arbitrarily divided by 1/2 to get 0.5ppm of Arsenic that was used for chronic dosage treatment.
