Biological triplicate analyses of HaCaT cells not exposed to arsenic (passage control) and HaCaT cellexposed to0.5 ppm arsenic for 8 passages, (passage in this study means sub-culturing after confluence). Ctrl=Control (no arsenic exposure, passage control) while Ars=Arsenic exposure (0.5ppm of arsenic for up-to 8 passages). Proteins were extracted from both exposed and unexposed HaCaT cell samples. An aliquot of protein lysate from each of the 6 culture plates was collected and resolved by SDS-PAGE/Coomassie. Each of the 6 gel lanes was entirely sliced into 24 bands, subjected to trypsin digestion, and peptides were analyzed by LC-MS/MS with a Thermo LTQXL mass spectrometer equipped with an Eksigent nano2D-LC. Proteins were identified with Mascot (95% confidence threshold). Pearson correlation analyses were performed to test reproducibility.
