Figure 2: FKBP51 downmodulation of melanoma reduces levels of active PKC. Western blot assay of pPKC and FKBP51 levels in cell lysates obtained from two different melanoma cell lines, transfected with a specific FKBP51 siRNA, or a non-silencing (NS) RNA as control. Cell was harvested after 24 h from transfection, for lysate preparation.