Figure 3: Cooperative effect of enzastaurin and ionizing radiation in inducing melanoma cell death. A: Graphic representation of cell death values (columns are means and bars standard deviations) of SAN melanoma cells. Cells were irradiated with a 4 Gy dose and incubated in the absence or the presence of 0.6 μM and 2 μM enzastaurin for 48 h. Cell death was measured by annexin-V staining. Data were obtained from three independent experiments, each performed in triplicate. B: Flow cytometric histograms of active Caspase 3, of SAN melanoma cells treated with 4 Gy IR and/or 2 μM enzastaurin. Cells were harvested after 6h to measure caspase activity. Result is representative of two independent experiments.