Figure 5: Enzastaurin reduced number of ABCG2+ cells in irradiated cultures. Graphic representation of ABCG2+ cell counts in A375 melanoma cultures, irradiated or not with a 4 Gy dose and incubated in the absence or the presence of 2 μM enzastaurin. After 72 h, cells were harvested, stained with anti-ABCG2-PE and acquired with a flow cytometer. A gate was placed on live cells on the basis of the physical parameters FSC/SSC; the number of ABCG2+ cells was calculated and expressed as number of positive cells/104 total cells. Data were obtained from four different experiments, each performed in triplicate.