Figure 1: SDS-PAGE analysis of the recombinant Glutamine Binding Protein in E. coli and purified by affinity chromatography. The Glutamine Binding Protein is evident (arrow) in the samples obtained after elution. M, molecular markers (expressed in kDa), T1 and T2, cell lisates after 6 and 10 hours induction; UB, portion of cell lysate not bound to the affinity resin; W, column wash buffer to eliminate aspecificities; lanes 1-5, fractions 1-5 eluted from the affinity column.