Figure 2: Western blot analysis of the recombinant Glutamine Binding Protein in E. coli purified by affinity chromatography. M, molecular markers (expressed in kDa), T1 and T2, cell lisates after 6 and 10 hours induction; UB, portion of cell lysate not bound to the affinity resin; W, column wash buffer to eliminate aspecificities; lanes 1-5, fractions 1-5 eluted from the affinity column. Glutamine Binding Protein was revealed by a commercial anti-histidine antiserum.