Figure 5: Western Blot analysis of wheat flour treated with trypsin, chymotrypsin and trypsin+chymptrypsin detected by recombinant GlnBP. A: G, recombinant GlnBP; wheat flour digested at 37°C for 10 min with 1) trypsin, 2) chymotrypsin and 3) trypsin+chymotrypsin; WF, wheat flour incubated in buffer for 10 min at 37°C. B: G, recombinant GlnBP; wheat flour digested at 25°C for 10 min with 1) trypsin, 2) chymotrypsin and 3) trypsin+chymotrypsin; WF, wheat flour incubated in buffer for 10 min at 25°C. Recombinant GlnBP was probed against an anti-histidine antiserum.