Figure 6: Western Blot carried out on wheat and corn flour after digestion with chymotrypsin at 25°C for 10 min. A: G, recombinant GlnBP; W1 (wheat flour) and C1 (corn flour) incubated in buffer at 25°C for 10 min; W2 (wheat flour) and C2 (corn flour) digested with chymotrypsin at 25°C for 10 min, revealed by the recombinant GlnBP. B: W1 (wheat flour) and C1 (corn flour) incubated in buffer at 25°C for 10 min; W2 (wheat flour) and C2 (corn flour) digested with chymotrypsin at 25°C for 10 min, revealed by the 4F3 monoclonal antibody. C: W1 (wheat flour) and C1 (corn flour) incubated in buffer at 25°C for 10 min; W2 (wheat flour) and C2 (corn flour) digested with chymotrypsin at 25°C for 10 min, revealed by the R5 antibody.