Figure 8: Schematic diagram of the protein microarray. Each subarray was spotted in triplicate with gliadin digested with chymotrypsin (Digested Gliadin) and with untreated gliadin (Gliadin), incubated in buffer for 10 min at 25°C. BSA was used as negative control (BSA). The proteins were immobilized in three different concentrations 500 μg/ml (subarray 1 and 4), 50 μg/ml (subarray 2 and 5) and 5 μg/ml (subarray 3 and 6).