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Figure 9: Acquired image by array scanner of subarrays where digested and untreated gliadin were immobilized in triplicate according to the diagram in Figure 8 at the concentration of 500 μg/ml (subarrays 1 and 4), 50 μg/ml (subarrays 2 and 5) , and 5 μg/ml (subarrays 3 and 6). The subarrays were incubated with A) recombinant GlnBP and B) 4F3 monoclonal antibody both labeled with Alexa-Fluor 555. |