Epub Date Source Enrichment Method Identified Proteins Identification Confidence Quantitation Method EXP/CON cutoff Candidate S-acylproteins Reference
06/02/2006 Yeast Saccharomyces cerevisiae ABE 1,557 ≥ two peptides (XCorr ≥ 1.8 (+1), 2.5 (+2), and 3.5 (+3), and ΔCn ≥ 0.08) SC 5.5 70 [14]
10/30/2007 Rat liver mitochondria MLCC (azido-palmitate) 21 Peptide mass fingerprinting (p<0.05) Gel band intensity n/a 21 [11]
12/18/2008 Rat embryonic cortical neurons and synaptosomes ABE 1,647 (neurons); 1,337 (synaptosomes) ≥ two peptides (XCorr ≥ 1.8 (+1), 2.5 (+2), and 3.5 (+3), and ΔCn ≥ 0.08) SC Graphic analysis 163 (high)a)
332 (medium)b)
[20]
01/11/2009 Human Jurkat T cells MLCC (17-ODYA) n/a ≥ two peptides (XCorr ≥ 1.8 (+1), 2.5 (+2), and 3.5 (+3), and ΔCn ≥ 0.08) SC 5 (high)
2-5 (medium)
125 (high)
199 (medium)
[12]
10/02/2009 Human prostate cancer DU145 cells ABE (PalmPISC) 928 ≥ two different peptides (p<0.05) SC 6.7 (high)
3.0-6.7 (medium)
169 (high)
224 (medium)
[21]
07/04/2010 Mouse dendritic DC2.4 cells MLCC (17-ODYA) n/a n/a SC 10 (high)
5-10 (medium)
60 (high)
97 (medium)
[22]
11/14/2010 Human Jurkat T cells MLCC (five az/alk fatty acid analogs) n/a ≥ two peptides (p<0.05) SC 5 (high)
3-5 (medium)
178 (high)
194 (medium)
[23]
12/30/2010 Parasite Trypanosoma brucei ABE 1,672 FDR=0.016 SC 2  and QSpec FDR=0.01 124 [24]
04/03/2011 Human cervical cancer HeLa cells MLCC (HDYOA) 53 n/a SC n/a n/a [13]
07/23/2011 Mouse RAW 264.7 macrophages ABE 1,183 FDR<0.01 (≥ two unique peptides) SC n/a 80 (FDR=0.05); 21 (FDR=0.10-0.05) [25]
08/02/2011 Human platelets ABE (PalmPISC) 1,300 FDR<0.01 SC 3 215 [26]
11/06/2011 Mouse T-cell hybridoma cells derived from BW5147 MLCC (17-ODYA) n/a FDR<0.01 SILAC2plex 1.5 415 (PAc) control)
338 (Hydd) control)
338 (both)
[27]
11/11/2011 Mouse neural stem cells MLCC (17-ODYA) n/a Peptide FDR<0.01 SILAC2plex 5 434 (PA control)
940 (Hyd control)
~300 (both)
[28]
04/10/2012 Human endothelial EA.hy926 cells ABE n/a n/a No No 154 [29]
05/17/2012 Human B lymphoid cells ABE 493 FDR<0.01 SC Spectral index>0.54 139 [30]
08/16/2012 Parasite Plasmodium falciparum ABE and MLCC (17-ODYA) 1,752 FDR<0.01 SILAC2plex n/a 353 (ABE), 176 (MLCC) [31]
12/17/2012 Arabidopsis root-derived callus cells ABE 924 FDR<0.01 iTRAQ4plex 1 144 (high)
115 (medium)
[32]
01/01/2013 Mouse epididymal fat pads and 3T3-L1 adipocytes ABE (Acyl-TPC) 856 ≥ three unique peptides No No 856 [19]
07/02/2013 Yeast Schizosaccharomyces pombe MLCC (17-ODYA) n/a n/a SC 2 n/a (>238) [33]
11/07/2013 Mouse brain ABE ~300 FDR<0.02 (≥ 40 peptides) No No ~300 [34]
12/19/2013 Human umbilical vein endothelial cells ABE ~1,700 n/a SILAC2plex 1.5 ~500 [35]
a): High-confidence; b): Medium-confidence; c) Palmitate; d) Hydroxylamine.
Table 1: Summary of global profiling studies of S-acylated proteins.