Figure 4: Data evaluation of positive and negative peak signals with Achroma ‘signal recognition’ module is exemplarily demonstrated by iAP assay with AMP substrate and injections of different ADP concentrations. EICs of Ado (A), AMP (B) and ADP (C) are presented. Each ADP concentration was injected twice, resulting in trace responses of all assay components (framed areas). Positive peak areas (AMP, ADP) and negative peak areas (Ado) are furthermore displayed as positive and negative bars with their respective standard deviations out of n=4 (D). The data evaluation interface is divided into functional subdivisions: selection of m/z range of interest with specified intensity minimum (I), automatic detection of positive or negative peak signals respectively (II), further options to edit data like smoothing of EICs, signal calculation with smoothed graph and displaying of automatically calculated peak areas (III), manual adjustment of peak starting and end points and possibility to include or exclude non-detected or false detected peaks respectively (IV).