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Figure 6: ‘Spectra comparison’ interface of the Achroma software and data evaluation of signal peaks: Injection of 80 μM (A, left dashed line) and 2 mM ADP (A, right dashed line) with no injection baseline time points (A, dark solid lines); difference spectrum of 80 μM ADP injection (B); difference spectrum of 2 mM ADP injection (C). AMP and ADP appeared as positively charged ions, either with proton, sodium or potassium. Ado was solely detected as protonated ion. |