Protein/Gene Name Gene Index Predicted Function/Domain Mol. Wt. (kDa) Pep. Id. Aa. Seq. Cov. (%)
General stress protein gsp-781* SGO_2107 Unknown 41 211 63
Glucosyltransferase G gtfG* SGO_0497 Glycosylhydrolase 178 175 62.8
Serine protease challisinsgc* SGO_0566 Serine protease 165 131 52.2
Serine protease degP* SGO_2150 Serine protease 42 102 43.6
Surface-associated protein cshA* SGO_0854 Unknown 264 85 40.1
Streptococcal surface protein A sspA* SGO_0210 Unknown 172 75 38.3
Surface antigen SCP-like domain SGO_1110 Unknown 82 72 46.2
Membrane protein SGO_0060 Membrane protein 109 66 41.8
5'-nucleotidase family protein* SGO_1247 N-terminal metallophosphatase domain 78 64 55.6
Streptococcal surface protein B sspB* SGO_0211 Unknown 164 58 38.7
LPXTG cell wall surface protein* SGO_0707 Cell wall surface protein 180 52 54.6
LPXTG cell wall surface protein* SGO_1651 Cell wall surface protein 84 50 37.9
Surface-associated protein cshB* SGO_1148 Unknown 244 46 32.9
Penicillin-binding protein 1B pbp1B SGO_1928 Transpeptidase domain 92 34 43.6
Exo-beta-D-fructosidase* SGO_0385 Glycosyl hydrolases family 32 156 32 29.9
LPXTG cell wall surface protein* SGO_0890 Collagen binding domain 69 32 22.7
Zinc metalloproteinase B zmpB* SGO_0408 IgA1-specific Metallo-endopeptidase N-terminal region 218 28 18.2
Putative transcriptional regulator lytR* ∞ SGO_0535 Membrane bound transcriptional regulator 44 28 44.2
Lipoprotein* SGO_1082 Lipoprotein 37 28 70.2
LPXTG cell wall surface protein* SGO_1487 Cna protein B-type domain 191 28 50.7
Putative N-acetylmuramidase*   SGO_2013 Glycosyl hydrolases family 25 127 28 33.4
Lipoprotein* SGO_0094 Unknown 42 26 43.1
Putative uncharacterized protein SGO_1066 Unknown 49 26 53.9
Conserved domain protein* SGO_0021 Unknown 20 25 60.4
Putative uncharacterized protein* SGO_2136 Unknown 48 25 47.2
Putative uncharacterized protein SGO_0319 Unknown 32 23 26.1
LysM domain protein* SGO_0138 Involved in binding peptidoglycan 40 21 17.7
Oligopeptide-binding protein* SGO_1716 ABC-type oligopeptide transport system 73 20 35.7
LPXTG cell wall surface protein* SGO_0388 Zinc carboxypeptidase 120 19 22.3
LPXTG cell wall surface protein* SGO_0430 Unknown 96 19 35.3
Serine/threonine protein kinase SGO_0600 Serine/threonine protein kinase 67 18 24.6
Maltose/maltodextrin-binding protein* SGO_0104 Maltose binding domain 45 17 44.2
Oligopeptide-binding lipoprotein hppH* SGO_1715 ABC-type oligopeptide transport system 72 17 32.2
Protein with prophage function domain SGO_0067 Prophage function domain 52 16 35
Glyceraldehyde-3-phosphate dehydrogenase gap SGO_0207 Glycolysis 36 15 41.1
Transport protein SGO_0767 TroA like transporter domain 32 14 47.8
LPXTG cell wall surface protein* SGO_1650 Collagen binding domain 77 14 18.7
Pneumococcal histidine triad A protein SGO_1313 Unknown 124 13 13%
Autolysin, N-acetylmuramidase-like protein SGO_1138 Unknown 26 12 40.2
Sulfatase* SGO_1377 Sulfatase 83 12 26.3
LPXTG cell wall surface protein SGO_2005 Unknown 397 12 4.9
LPXTG cell wall surface protein* SGO_0107 Collagen binding domain 115 11 10.9
Amylase-binding protein B abpB* SGO_0162 Amylase-binding protein 73 11 25.5
Lipoprotein* SGO_0181 Unknown 36 11 36.7
Cell wall binding protein* SGO_0477 Glucan-binding domain 36 11 42.4
Peptidoglycan N-acetylglucosaminedeacetylase A pgdA* SGO_0948 Peptidoglycan N-acetylglucosaminedeacetylase 54 11 21.1
Penicillin-binding protein 2B pbp2B SGO_1449 Transpeptidase domain 75 11 22.8
Oligopeptide-binding lipoprotein hppG* SGO_1713 ABC-type oligopeptide transport  74 11 28.5
Lipoprotein* SGO_0068 Unknown 20 10 42.4
Lipoprotein hlpA* SGO_0458 ABC-type metal ion transport system 32 9 29.6
Cell wall binding protein* SGO_0478 Glucan-binding domain 37 9 38
Substrate-binding protein msmE* SGO_1305 Sugar transport 49 9 35.9
Branched-chain amino acid ABC transporter* SGO_1630 Amino acid-binding protein 41 9 25.3
Conserved domain protein* SGO_0018  Transglycosylase-like domain 21 8 25.5
Elongation factorTutuf SGO_0761 Protein translation 44 7 23.4
Enolaseeno SGO_1426 Glycolysis 47 7 17.1
Cell wall binding protein* SGO_0845 Glucan-binding domain 34 7 12.8
Cell shape-determining protein mreC* SGO_2108 Rod shape-determining protein 31 7 29.6
Penicillin-binding protein 1A pbp1a* SGO_0586 Penicillin binding protein transpeptidase domain 79 6 11.7
Putative uncharacterized protein SGO_0591 Unknown 50 6 10.5
SCP-like extracellular protein* SGO_0847 SCP-like extracellular protein domain 56 6 14.9
Putative uncharacterized protein SGO_0332 Unknown 53 5 15.6
Amino acid ABC transporter* SGO_0982 Amino acid-binding protein domain 31 5 18.7
Foldase protein prsA* SGO_1572  PPIase domain 34 4 18.8
Penicillin-binding protein 2X pbp2X* SGO_0575 Penicillin binding protein transpeptidase domain 83 4 5.7
Efflux transporter, RND family, MFP subunit family* SGO_0750 RND family efflux transporter 42 4 18.3
Lipoprotein* SGO_1165 Unknown 33 4 21.6
Sortase A srtA* SGO_1230 Cell wall anchoring of LPXTG proteins (Nobbs, 2007) 28 4 8.8
LPXTG cell wall surface protein* SGO_1415 X-prolyldipeptidylaminopeptidase 117 4 7.6
Peptidyl-prolylcis-trans isomerase* SGO_1463 Peptidyl-prolylcis-trans isomerase 29 4 12.7
Metal ABC transporter substrate-binding lipoprotein* SGO_1802 Metal ABC transporter 35 4 22.6
Elongation factor G fusA SGO_0206 Protein translation 77 3 10.2
Chaperone protein dnaK SGO_0402 Protein folding 65 3 4.9%
L-lactate dehydrogenase ldh SGO_1232 L-lactate dehydrogenase 35 3 9.1
Putative lipoprotein* SGO_0004 Unknown 20 3 17.4
Possible cell wall protein* SGO_0846 SCP-like extracellular protein domain
n 		76 3 5.2
Glutamine ABC transporter permease and substrate binding protein* SGO_1037 ABC transporter 53 3 6.4
Thioredoxin family protein SGO_1171 Thioredoxin 18 3 31.2
D-Alanyl-D-Alanine carboxypeptidase* SGO_1585  D-alanyl-D-alanine carboxypeptidase 51 3 6.7
5'-nucleotidase, lipoprotein* SGO_1860 Haloaciddehalogenase-like hydrolases 32 3 11.2
Putative uncharacterized protein SGO_1870 Unknown 65 3 5.1
Putative uncharacterized protein* SGO_1932 Unknown 22 3 24.6
Lipoprotein* SGO_2038 Unknown 17 3 21.4
Lipoprotein* SGO_0227 Unknown 23 3 8.1
Arginine--tRNA ligase argS SGO_2058 Arginine--tRNA ligase 63 3 6.9
Putative peptidoglycan hydrolase and general stress protein* SGO_0212 Putative peptidoglycan hydrolase 23 3 12
Putative uncharacterized protein pXO1 SGO_0059 Unknown 11 2 35.8
Putative uncharacterized protein SGO_0080 Unknown 62 2 5.3
Putative acyltransferase* SGO_0112 Putative acyltransferase 68 2 3.5
Putative uncharacterized protein* SGO_0329 Unknown 69 2 5.4
Aminodeoxychorismatelyase-like protein SGO_0518 Unknown 60 2 3.3
Pyruvate kinase pyk SGO_1339 Glycolysis 55 2 7.4
ABC transporter, substrate-binding protein* SGO_1763 ABC transporter 54 2 7.6
LPXTG cell wall surface protein, glycosyl hydrolase* SGO_0208 Glycosyl hydrolase family 85 174 2 1.6
Lipoprotein* SGO_1931 Unknown 19 2 9.4
ABC transporter, substrate-binding protein* SGO_0457 ABC transporter 32 2 6.7
Putative uncharacterized protein SGO_0832 Unknown 13 2 26.3
Penicillin-binding protein 2A pbp2A SGO_2010 Penicillin binding protein transpeptidase domain 81 2 2.6
Penicillin-binding protein 3 pbp3* SGO_1717 Penicillin-binding protein domain 46 2 5.6
Elongation factorTstsf SGO_2000 Protein translation 37 2 7.2
Putative uncharacterized protein SGO_1756 Unknown 19 2 16.1
Zinc proteinase* SGO_2009 Zinc metalloprotease 34 2 7.9
Mol. Wt. kDa – Molecular weight in kDa (http://web.expasy.org/compute_pi/)
Pep. Id. – No. of peptides identified in MS/MS analysis
Aa. Seq. Cov. - % of the total aminoacid sequence covered by identified peptides
*Proteins with N-terminal signal for secretion (http://www.predisi.de/home.html)
Table 1: Whole secretome proteins identified in common from KS1 and ΔAbpA.