No Clade Gene Gene ID Chromosome location Start position in scaffold Genomic sequence length (bp) CDS sequence length (bp) Amino acid  sequence length (aa) No of exons CDS identity (%) Amino acid identity (%) Duplication time (MYA) Ks Catalytic site
C248 H416 N449 S307
1 I KCS1-1 Lus10006637 Scaffold244 (LG8) 250465 2,499 1,623 540 2 76.95 72.4 13.7 0.178 + + + +
2 KCS1-2 Lus10039401 Scaffold33 (LG10) 1236391 2,321 1,308 435 3                
3 KCS2-1 Lus10021895 Scaffold164 (LG3) 494574 3,092 1,425 474 2 85.57 85.79 7.1 0.092 + + + +
4 KCS2-2 Lus10041177 Scaffold280 (LG15) 1287373 3,083 1,584 527 2         + + + +
5 KCS3-1 Lus10019446 Scaffold906 (LG6) 285487 1,551 1,551 516 1 97.73 98.44 1.9 0.024 + + + +
6 KCS3-2 Lus10043300 Scaffold25 (LG12) 2296451 1,551 1,551 516 1         + + + +
7 KCS3-3 Lus10019448 Scaffold906 (LG6) 290816 1,343 1,281 426 2 89.87 83.13 1.7 0.022 + + + +
8 KCS4-1 Lus10002691 Scaffold1347 (LG15) 106658 1,748 1,572 523 2 92.93 95.21 14.1 0.183 + + + +
9 KCS4-2 Lus10030209 Scaffold217 (LG4) 231412 1,893 1,572 523 2         + + + +
10 KCS5-1 Lus10010108 Scaffold722 (LG1) 258593 1,575 1,575 524 1 96.06 98.28 4.9 0.063 + + + +
11 KCS5-2 Lus10012611 Scaffold101 (LG15) 126220 1,575 1,575 524 1         + + + +
12 KCS9-1 Lus10006636 Scaffold244 (LG8) 241675 1,974 1,482 493 2 81.37 77.51 23.3 0.302 + + + T
13 KCS9-2 Lus10039399 Scaffold33 (LG10) 1219216 1,811 1,524 507 2         + + + T
14 II (ε) KCS10 Lus10028105 scaffold132 (LG5) 1068330 1,678 1,518 505 3 88.50 90     + + + +
15   KCS11-1 Lus10016528 scaffold279 (LG12) 371666 2,781 1,587 529 3 96.84 97.73 6.0 0.078 + + + +
16   KCS11-2 Lus10040796 scaffold156 (LG3) 1494523 3,052 1,587 528 3         + + + +
17   KCS11-3 Lus10009799 scaffold1 186142 2,565 1,527 508 4 88.50 90 22.6 0.293 + + +  
18 III (α) KCS6-1 Lus10001657 scaffold227 (LG8) 14836 1,512 1,512 503 1 96.75 97.21 4.2 0.055 + + + +
19   KCS6-2 Lus10002533 scaffold81 (LG6) 60705 1,512 1,512 503 1         + + + +
20   KCS7-1 Lus10026345 scaffold898 (LG11) 718114 1,551 1,551 516 1 95.35 98.64 5.2 0.067 + + + +
21   KCS7-2 Lus10042318 scaffold123 (LG1) 1671369 1,548 1,548 515 1         + + + +
22 IV (γ) KCS8-1 Lus10034319 scaffold310 (LG1) 596075 1,491 1,491 496 1 95.7 98.79 6.3 0.082 + + + +
23   KCS8-2 Lus10041452 scaffold272 (LG4) 142994 1,491 1,491 496 1         + + + +
24 V KCS12-1 Lus10020662 scaffold303 (LG4) 74137 1,350 1,350 449 1 65.72 62.3 36.0 0.469 + + + +
25   KCS12-2 Lus10031486 scaffold863 523726 1,248 1,248 415 1         + + + +
26   KCS12-3 Lus10029880 scaffold416 487616 1,362 1,362 453 1 65.72 52.35 23.4 0.304 + + + +
27 VI(θ) KCS16-1 Lus10005434 scaffold847 (LG7) 119164 1,464 1,464 487 1 91.49 92.9 8.9 0.116 + + + T
28   KCS16-2 Lus10015222 scaffold924 (LG12) 18075 1,479 1,479 492 1         + + + T
29   KCS17-1 Lus10004918 scaffold858 (LG8) 122399 1,239 1,239 412 1 63.45 62.83 6.3 0.082 + + + T
30   KCS17-2 Lus10040873 scaffold156 1981104 1,188 1,188 395 1         + + + T
31 VII(θ) KCS13-1 Lus10033626 scaffold701 (LG12) 874027 2,211 1,455 484 4 68.71 65.36 14.9 0.194 + + + T
32   KCS13-2 Lus10033627 scaffold701 (LG12) 878209 1,722 1,122 373 3         + + + T
33   KCS13-3 Lus10033625 scaffold701 (LG12) 867459 5,336 2,271 756 4 49.28 44.28 12.0 0.156 + + + T
30   KCS14-1 Lus10002191 scaffold1010 67381 1,401 1,401 466 1 76.57 76.48 11.0 0.144 + + + T
31   KCS14-2 Lus10039906 scaffold15 (LG4) 2153982 3,627 1,725 574 2         + + + T
32   KCS15 Lus10002192 scaffold1010 69983 1,254 1,254 417 1         + + Y T
37   KCS18-1 Lus10023458 scaffold1216 (LG7) 288113 1,575 1,437 478 2 93.73 96.02 14.0 0.183 + + + T
38   KCS18-2 Lus10040333 scaffold86 1404742 1,560 1,437 478 2         + + + T
For genes with three duplicate members, the estimates of sequence identity, duplication time and Ks of the third member were calculated as the average of the first two members vs. the third one. Clade: the clades are determined according to the phylogenetic analysis of KCS genes (Figure 6). The clades in parentheses correspond to classification of KCS genes in Arabidopsis [42]. Catalytic site: +represents the presence of the residues of putative catalytic sites. The positions of residues, Cys248, His416, Asn449 and Ser307, are based on the sequence of KCS1-1.
Table 5: KCS gene family related to the fatty acid elongation to VLCFA identified in flax cv. CDC Bethune.