No Clade Gene Locus Chromosome location Start position in scaffold Genomic sequence length (bp) CDS sequence length (bp) Amino sequence length (aa) No. of exons CDS identity (%) Amino acid identity (%) Duplication time (MYA) Ks Catalytic site H275 C310 1 FatA FatA1-1 Lus10038190 scaffold28 (LG11) 210541 2,873 1,125 370 8 84.71 79.94 6.2 0.08 + + 2   FatA1-2 Lus10025912 scaffold605 (LG1) 914799 2,259 1,113 374 7         + + 3   FatA2-1 Lus10022772 scaffold8 (LG10) 41937 2,422 1,131 376 7 96.64 95.74 2.7 0.035 + + 4   FatA2-2 Lus10011839 scaffold754 (LG9) 29342 2,395 1,131 376 7         + + 5 FatB FatB1-1 Lus10017751 scaffold212 (LG6) 296331 2,015 1,257 418 6 97.93 98.80 0.5 0.006 + + 6   FatB1-2 Lus10033072 scaffold306 (LG12) 739174 2,024 1,257 418 6         + + 7 Fat I Fat1-1 Lus10013480 scaffold230 (LG3) 386737 2,394 1,257 418 6 92.91 93.54 3.4 0.044 + + 8   Fat1-2 Lus10007942 scaffold281 (LG3) 75576 2,338 1,251 416 6         + + 9   Fat2-1 Lus10034617 scaffold9 (LG13) 987681 1,889 1,230 409 6 94.89 93.41 2.2 0.029 + + 10   Fat2-2 Lus10000365 scaffold2160 5905 1,888 1,233 410 6         + + 11 Fat II Fat3-1 Lus10035901 scaffold76 (LG11) 200209 1,547 1,161 383 5 89.49 87.82 5.2 0.067 + + 12   Fat3-2 Lus10025762 scaffold605 (LG1) 301503 1,444 1,152 386 4         + + 13   Fat4-1 Lus10025763 scaffold605 (LG1) 304576 1,350 1,077 363 4 90.29 85.95 5.5 0.071 + + 14   Fat4-2 Lus10035900 scaffold76 (LG11) 197570 1,357 1,092 358 4         + + Clade: the clades are determined according to the phylogenetic analysis of FAT genes (Figure 7). Catalytic site: + represents the presence of the residues of putative catalytic sites. The positions of residues, His275 and Cys310, are based on the sequence of FatA1-1.

Table 6: FAT gene family involved in the fatty acid chain termination identified in flax cv. CDC Bethune.