Figure 1: Analysis of composition of microvesicles released by vascular smooth muscle cells in response to lysoPC. Vascular smooth muscle cells were grown to confluence, starved in medium without serum for 4 hours and treated with lysoPC (6.25 μg/ml) for 16 hours. VSMC-derived MPs were then isolated and the proteins were separated by SDS-PAGE, subjected to trypsin digestion and analyzed by mass spectrometry. (A) Coomassie staining of gel. (B) Summary of cellular component and (C) biological function for each protein identified on lysoPC-derived MPs from a representative experiment. (D) The presence of proteins identified in the proteomic analysis (ERM, annexin-2, cav-1, RhoA, and Rac) was confirmed by western blot in total extracts (TE) and microparticles (MP) of lysoPC-treated VSMC. Blots shown are representative of at least 3 independent experiments.