Figure 1: Protein profiling of bovine mammary epithelial cells treated with LCFA. The cells were treated with serum-deprivation overnight and subsequently with 400 µM oleate (lower panel) conjugated to 0.5% BSA or BSA alone (lower panel) for 24 h. Representative data of 2-DE according to the procedures shown in Materials and Methods. Green squares indicate significant changed protein spot assigned by PDQuest software. Red arrowhead and number indicate protein spot number identified by MS/MS ion search.