Figure 2: Identification of proteins changed by LCFA. (A) Representative data of MS/MS spectra are shown. Upper panel indicates the spectrum of Bos taurus Transgelin-2 peptides. Lower panel indicates that of Bos taurus Calponin-2. (B) The cells were treated with serum-deprivation overnight and subsequently with 400 µM oleate (lower panel) conjugated to 0.5% BSA or BSA alone (lower panel) for 24 h. After that, cell lysates were analyzed by western bloting using specific antibodies against KRT8, TAGLN2, ANXA2, YWHAZ, PPIA, ATP5B and actin. The Figure shows data from a representative experiment.