Figure 3: Predicted PKCs are actually activated by LCFA. (A) Prediction of biological pathway based on data sets of identified proteins obtained from public protein-protein interaction database, BioGrid. Red circles indicate identified protein, while pink circles indicate molecules, which can bind identified protein. The edges indicate physical interaction between two molecules. Red arrowhead indicates PKC activations, which is confirmed by this study. Black one indicates PKC activations, which is not confirmed by this study, otherwise that UCP2, which is reported to up-regulation by our previous study in transcriptional level, could not observe any change in microarray in this study. Yellow one indicates molecules, which are reported to activation by LCFA in our previous study. Blue one indicates molecules, which is significantly induced by LCFA in microarray in this study. (B) Cell lysates were separated as cytosolic and membrane fractions, and analyzed by western blotting using antibodies against each PKCs. The Figure shows data from a representative experiment. The rates of translocation are expressed as fold changes relative to control cells (membrane protein density normalized against those of the cytosolic fraction). The amounts of KRT17 are expressed as fold changes relative to control cells. The data are expressed as means ± SEM (n=3). Asterisk indicates significantly different (P<0.05, t-test).