Figure 3: SYPRO Ruby-stained 2DE gels (pH range 3-10) from the conditioned medium of non-treated ECPC-4 (Figure 3: left) and ECPC-4 treated with lipid A (500 ng/ml) (Figure 3: right) for 48 h. For detection of protein expression, 500 µg of protein was loaded to each gel. Protein molecular mass standards (y-axis) and pHs (x-axis) are shown.