Figure 4: Western Blotting of galectin-1, nucleoside diphosphate kinase A and proteasome subunit alpha type1 in the conditioned medium of non-treated and lipid A-treated ECPC-4. NT, medium of non-treated ECPC-4 conditioned for 48 h; LipA, medium of ECPC-4 with lipid A (500 ng/ml) conditioned for 48 h. GAL1: galectin-1, NME: nucleoside diphosphate kinase A, PSMA1: proteasome subunit alpha type1. SPARC was used as the internal control protein. (A) The levels of GAL1, NME, PSMA1 and SPARC in individual samples of each group were detected. (B) Graphical representation of the semi quantitative analysis. (mean ± S.E.M. of O.D. of bands). Data are presented as mean ± S.E.M. of 3 samples from each group.