Spot number GI accession number Identified protein name Theoretical Mass/PI Score Sequence coverage Number of peptides Functions/ Cellular localization
1 309533 Vinculin 117303/5.72 66 5 3 intermediate filament C
2 70778915 Moesin 67839/6.22 461 12 8 cytoskeleton C
3 13242237 Heat shock cognate 71 kDa protein 71055/5.37 308 14 6 protein folding C
4 6680836 Calreticulin precursor 48136/4.33 187 11 4 signal transduction C
5 4507729 Tubulin beta-2A chain 50274/4.78 113 8 3 cytoskeleton C
6 497775 Fascin 55112/6.21 74 3 1 intermediate filament C
7 129577 Plasminogen activator inhibitor 1 45198/6.17 164 18 6 signal transduction S
8 6678077 SPARC precursor 35283/4.77 269 19 5 cell growth S
9 809561 Gamma-actin 41335/5.56 223 15 4 cytoskeleton C
10 10946870 Alcohol dehydrogenase 36792/6.9 107 12 3 metabolism C
11 387129 Cytosolic malate dehydrogenase 36625/6.16 117 8 3 metabolism C
12 10179944 Phosphoglyceratemutase 1 29033/6.19 60 21 3 metabolism C
13 13435747 Rho GDP dissociation inhibitor alpha 23434/5.12 106 23 3 signal transduction C
14 6678437 Translationally-controlled tumor protein 19564/4.76 209 21 4 cytoskeleton C
15 84794552 Phosphatidylethanolamine-binding protein 1 20988/5.19 247 36 5 signal transduction M
16 3603241 Type IIperoxiredoxin 1 21949/5.2 64 18 5 redox regulation C
17 6754976 Peroxiredoxin-1 22390/8.26 76 14 3 redox regulation C
18 6680924 Cofilin-1 18776/8.22 64 18 2 cytoskeleton C
19 6679439 Peptidyl-prolylcis-trans isomerase A 18131/7.74 91 21 2 metabolism C
20 37700232 Nucleoside diphosphate kinase A 17311/6.84 145 38 5 metabolism C
21 17986258 Myosin light polypeptide 6 isoform 1 17090/4.56 77 13 2 cytoskeleton C
22 6678682 Galectin-1 15198/5.32 337 39 5 signal transduction C
The proteins of conditioned medium were separated by 2DE and identified by MALDI-TOF MS/MS, following in-gel digestion with trypsin. The spots representing the identified proteins are indicated in Figure 2 and are designated by gene ID accession numbers (GI acc. no.) in the Swiss Prot database. Score relates to the probability assignment, theoretical molecular weight, and pI, and sequence coverage (SC) values are given. Score and sequence coverage were calculated via the MASCOT search engine (http://www.matrixscience.com). Note that all MASCOT scores are greater than 60 (p<0.05). Cellular localization of proteins is also indicated (S, secreted; C, cytosolic; M, membrane).
Table 1: Proteins identified in the supernatant of ECPC-4 cell cultures.