Spot number GI accession number Identified protein name Theoretical Mass / PI Score Sequence coverage Number of peptides Fold increase / decrease Functions Cellular localization 1 33563282 Proteasome subunit alpha type-1 29813/6 70 9 2 1.3 proteolysis C 2 809561 Gamma-actin 41335/5.56 160 10 3 0.77 cytoskeleton C 3 809561 Gamma-actin 41335/5.56 223 15 4 0.77 cytoskeleton C 4 37700232 Nucleoside diphosphate kinase A 17311/6.84 145 38 4 1.2 metabolism C 5 6678682 Galectin-1 15198/5.32 235 33 4 0.83 signal transduction C 6 809561 Gamma-actin 41335/5.56 221 15 3 0.83 cytoskeleton C 7 6678682 Galectin-1 15198/5.32 337 39 4 0.83 signal transduction C

The proteins of conditioned medium were separated by 2DE and identified by MALDI-TOF MS/MS, following in-gel digestion with trypsin. The spots representing the identified proteins are indicated in Figure 3 and are designated by gene ID accession numbers (GI acc. no.) in the Swiss Prot database. Score relates to the probability assignment, theoretical molecular weight, and pI, and sequence coverage (SC) values are given. Score and sequence coverage were calculated via the MASCOT search engine (http://www.matrixscience.com). Data were analyzed by ANOVA (p<0.05). Cellular localization of proteins is also indicated (S, secreted; C, cytosolic; M, membrane).

Table 2: Differentially expressed proteins in the supernatant of lipid A-treated ECPC-4 cell cultures when compared with untreated (n=3).