Figure 3: Validation of IGK@ protein, HSPβ-1, Apo-A1and RPLP2 by western blot analysis in fertile (LH+7) and infertile (LH+7) human endometrial biopsy samples which had not been previously analyzed in the 2D-PAGE analysis. The results matched the previous ones. Representative images (upper panel) of immunoblot of IGK@ protein, HSPβ-1, Apo-A1and RPLP2 have been shown here. β-actin served as a loading control for normalization. Quantification of band intensity (lower panel) was performed by densitometric analysis by using Quantity One® software (v. 4.5.1) and a gel doc imaging system (Bio-Rad). P values are (b) p<0.01 and (c) p<0.05 versus fertile group. Values are expressed as mean± SE; n=8.