Figure 5: Western blot analysis of IGK@ protein, Apo-A1, RPLP2 and Prolactin during in-vitro decidualization of hESCs. Expression of decidual marker prolactin was checked in hESCs and decidualized hESCs (A). Representative images of immunoblot of IGK@ protein, Apo-A1and RPLP2 have been shown here (A). β-actin served as a loading control for normalization. Quantification of band intensity (B, C) was performed by densitometric analysis by using Quantity One® software (v. 4.5.1) and a gel doc imaging system (Bio- Rad). P values are (a) p<0.001, (b) p<0.01 and (c) p<0.05 versus hESCs. hESCs, human endometrial stromal cells; Dec-hESCs, Decidualized human endometrial stromal cells. Values are expressed as mean ± SE; n=3.