Figure 3: Expression of LPS-stimulated moesin in HGF. HGF was cultured in 6-well dishes and stimulated with LPS from P. gingivalis treatment for 24 hrs. Total cell lysates were prepared and separated by electrophoresis on a 10–20% polyacrylamide gradient gel and immunoblotted with the anti-moesin antibody. The intensity of each band was measured and the moesin protein levels normalized with β-actin were calculated. The intensity of each band was measured by imaging analysis. Data are expressed as the mean ± SD of the number of replicates (n). Results shown are from three independent experiments. The moesin band volumes were significantly increased in the severe periodontal disease group when compared with those in the control group (p<0.05).