| Gene |
Primer Name |
Oligomer |
Product Size (bp) |
Temperature (°C)/time (sec)
Denaturation Annealing Elongation |
| Putative pathogenesis related protein |
PR |
Forward: TGAGTCCACAACCACA
Reverse: GCGAGGAGGTACGCTTCGA |
450 |
95/30 |
52/30 |
72/60 |
| Pathogenesis-related protein 10 |
PR10 |
Forward: CCACAGCCTCAGTTGCCCCA
Reverse:GGCGAGGAGGTATGCTTCGATGGC |
432 |
95/30 |
57/30 |
72/60 |
| NACA3 |
NACA3 |
Forward: CTCGCCGCCAAATTGGAAGA
Reverse:CACGTTGCTGAGGTTGGGAGC |
387 |
95/30 |
52/30 |
72/60 |
| Triose phosphate isomerase cytosolic isoform-like |
TPI |
Forward: TGCAACCCCTGCACAAGCCC
Reverse: GGCGCCTTTCTTCACCTCAGC |
235 |
95/30 |
54/30 |
72/60 |
| Unknown |
Unkown |
Forward: GGCCACTTTGCCCGTTCCAAT
Reverse:CCATGGCAGGCACCGGCAAG |
533 |
95/30 |
54/30 |
72/60 |
| Enolase-like |
ENOL |
Forward: TCAAAATGAGTGGGGTTGGTGCAA
Reverse: AACCACCCTCGTCACCGACA |
353 |
95/30 |
54/30 |
72/60 |
| LEXYL2 |
LEXYL2 |
Forward: CACGGCAGGTTCATTGCCTCT
Reverse:TGCAGCTGCTACTTTCTTGGCA |
229 |
95/30 |
52/30 |
72/60 |
