Spot No. Protein Identity Pfam match Gene Ontologya Localizationb
Nucleotide transport and metabolism (F)      
1 Nucleoside diphosphate kinase (gi|12230332) Nucleoside diphosphate kinase Transcription, DNA-dependent, DNA binding, pyridoxal phosphate binding; sequence-specific DNA binding transcription factor activity Cytosol,  Mitochondria, Nucleus
Post translational modification, protein turnover (O) / Energy production and conversion (C)  
2 Thioredoxin H-type 1 (gi|267124)  Thioredoxin Cell redox homeostasis, electron transport chain, glycerol ether metabolic process, electron carrier activity; protein disulfide oxidoreductase activity Cytosol
101 Protein disulfide isomerase-like protein (gi|49257109) Thioredoxin Cell redox homeostasis, glycerol ether metabolic process, electron carrier activity; protein disulfide oxidoreductase activity Endoplasmic reticulum
4 Hypothetical protein VOLCADRAFT_120809 (gi|302836253) Thioredoxin Cell redox homeostasis, glycerol ether metabolic process, electron carrier activity; protein disulfide oxidoreductase activity Chloroplast
Translation, ribosomal structure and biogenesis (J)  
3, 7 Constitutive plastid-lipid associated protein (gi|75266239) Endoribonuclease L-PSP No related GO Chloroplast
10 Unknown (gi|118482257) Ribosomal protein family No related GO Nucleus
11 Eukaryotic translation initiation factor 5A-2 (gi|20138707) Eukaryotic elongation factor 5A hypusine, DNA-binding OB fold Peptidyl-lysine modification to hypusine, positive regulation of translational elongation, ribosome binding,  translation elongation factor activity, translation initiation factor activity Chloroplast
Transcription (K)      
6, 8 Glycine-rich RNA-binding protein RGP-1c (gi|45533923) RNA recognition motif Nucleic acid binding, nucleotide binding Chloroplast
29 NACA3 (gi|240256288) NAC domain Protein transport, response to salt stress Nucleus
87 Actin-51 (gi|3219772) Actin ATP binding Cytosol
Inorganic ion transport and metabolism (P)    
9 Superoxide dismutase [Cu-Zn], chloroplastic (gi|134682) Copper/zinc superoxide dismutase (SODC) Superoxide metabolic process, metal ion binding, superoxide dismutase activity. Chloroplast, Cytosol, Mitochondria
15 Putative Cu/Zn superoxide dismutase (gi|171854653) Copper/zinc superoxide dismutase (SODC) Superoxide metabolic process, metal ion binding, superoxide dismutase activity Chloroplast
19 Copper chaperone (gi|15228869) Heavy-metal-associated domain Intracellular copper ion transport, oxidation-reduction process, superoxide dismutase copper chaperone activity, zinc ion binding Cytosol, Mitochondria,  Nucleus
48 L-ascorbate peroxidase (gi|804973) Peroxidase Embryo development ending in seed dormancy, hydrogen peroxide catabolic process, response to cadmium ion, heat / salt stress, L-ascorbate peroxidase activity, heme binding, metal ion binding Peroxisome
50 Cytosolic ascorbate peroxidase (gi|62910196) Peroxidase Response to oxidative stress, heme binding, peroxidase activity Peroxisome
Lipid metabolism (I)      
12 DH putative beta-hydroxyacyl-ACP dehydratase (gi|193290688) FabA-like domain Fatty acid biosynthetic process, lipid A biosynthetic process, hydro-lyase activity. Chloroplast
Posttranslational modification, protein turnover, chaperones (O)  
17 Unknown (gi|118481397) Redoxin No related GO Chloroplast
22 Thioredoxin peroxidase (gi|18654477) Redoxin Response to cadmium ion, peroxidase activity, peroxiredoxin activity Cytosol
24 Phospholipid hydroperoxide glutathione peroxidase (gi|31872080) Glutathione peroxidase Response to oxidative stress, glutathione peroxidase activity, phospholipid-hydroperoxide  glutathione peroxidase activity Chloroplast, Mitochondria
31 Unknown (gi|255641541) Proteasome subunit A No related GO Nucleus
33 Proteasome subunit beta type, putative (gi|255558626) Proteasome subunit Proteolysis involved in cellular protein catabolic process, threonine-type endopeptidase activity Nucleus
35 Proteasome subunit beta type-1 (gi|17380185 ) Proteasome subunit Proteolysis involved in cellular protein catabolic process, regulation of plant-type hypersensitive response, response to salt stress, response to zinc ion, peptidase activity; threonine-type endopeptidase activity Nucleus
36 Dehydroascorbate reductase (gi|160347100) Glutathione S-transferase, Response to oxidative stress Cytosol
39 Chaperonin 10 (gi|3057150) Chaperonin 10 Kd subunit Protein folding;  response to heat, ATP binding, copper ion binding Chloroplast
42 GSTL2 (gi|15233164) Glutathione S-transferase, Protein glutathionylation, response to toxin, glutathione transferase activity. --
44 Chaperonin 21 precursor (gi|7331143) Chaperonin 10 Kd subunit Protein folding, ATP binding Chloroplast
45 Unknown (gi|77416969) Proteasome subunit A N-terminal signature Ubiquitin-dependent protein catabolic process, threonine-type endopeptidase activity Nucleus
46 Proteasome-like protein alpha subunit (gi|77999303) Proteasome subunit A N-terminal signature Ubiquitin-dependent protein catabolic process, threonine-type endopeptidase activity Cytosol, Nucleus
56 Hypothetical protein VITISV_014475 (gi|147856362) Proteasome subunit A N-terminal signature Response to arsenic-containing substance, ubiquitin-dependent protein catabolic process, threonine-type endopeptidase activity. Nucleus
68 DegP protease precursor (gi|2565436) Trypsin-like peptidase domain Photosystem II repair, protein catabolic process, proteolysis, response to stress, serine-type endopeptidase activity Chloroplast
102 RuBisCO large subunit-binding protein (gi|1351030) TCP-1/cpn60 chaperonin family Protein refolding, ATP binding Chloroplast
105 Unknown (gi|116787373) TCP-1/cpn60 chaperonin family Protein refolding, ATP binding Chloroplast
106 RuBisCO large subunit-binding protein (gi|2506277) TCP-1/cpn60 chaperonin family Protein refolding, ATP binding Chloroplast
107 Stromal 70 kDa heat shock-related protein (gi|1708311) Hsp70 protein Protein folding, response to stress, ATP binding. Chloroplast
Energy production and conversion (C)      
27 ATP synthase subunit delta' (gi|2493046) ATP synthase, Delta/Epsilon chain ATP synthesis coupled proton transport, hydrogen ion transporting ATP synthase activity, rotational mechanism, proton-transporting ATPase activity, rotational mechanism Mitochondria
97 to 100 ATP synthase subunit beta, (gi|114421) ATP synthase F1 beta subunit ATP hydrolysis coupled proton transport, ATP synthesis coupled proton transport, ATP binding; hydrogen ion transporting ATP synthase activity, rotational mechanism, hydrogen-exporting ATPase activity, phosphorylative mechanism Chloroplast, Mitochondria
54 Predicted protein (gi|224103823) Inorganic pyrophosphatase Defense response to bacterium, phosphate-containing compound metabolic process, inorganic diphosphatase activity, magnesium ion binding Chloroplast, Cytosol
59 to 62 Malate dehydrogenase (gi|68299213) Lactate/malate dehydrogenase Carbohydrate metabolic process, malate metabolic process, L-malate dehydrogenase activity Chloroplast
64 Putative pyruvate dehydrogenase E1 beta subunit (gi|193290724) Transketolase, pyrimidine binding domain Defense response to bacterium, catalytic activity Chloroplast
72 Hypothetical protein (gi|302795987) Transketolase, pyrimidine binding domain Defense response to bacterium, catalytic activity Mitochondria
73 Predicted protein (gi|224053535) Transketolase, pyrimidine binding domain Defense response to bacterium, catalytic activity Mitochondria
89 Malate dehydrogenase (gi|56562183) lactate/malate dehydrogenase, NAD binding domain Carbohydrate metabolic process, malate metabolic process, malate dehydrogenase (NADP+) activity; nucleotide binding. Chloroplast
91 Putative branched-chain alpha-keto acid dehydrogenase (gi|193290668) Biotin-requiring enzyme Fatty-acyl-CoA biosynthetic process,
dihydrolipoyllysine (2-methylpropanoyl) transferase activity
Mitochondria
General function prediction only (R)      
30 Acidic 27 kDa endochitinase (gi|544010) Chitinase class I Cell wall macromolecule catabolic process, chitin catabolic process, defense response, chitinase activity Vacuole
85 Ankyrin-repeat protein HBP1 (gi|13310811) Ankyrin repeats (3 copies) No related GO Endoplasmic reticulum
Carbohydrate transport and metabolism (G)    
37, 38 Triose phosphate isomerase cytosolic  isoform (gi|38112662) Triosephosphate isomerase Glycolysis,  triose-phosphate isomerase activity Chloroplast
43, 47 Unknown (gi|255645535) Triosephosphate isomerase Glycolysis, triose-phosphate isomerase activity Chloroplast
79, 81 Enolase-like (gi|82623425) Enolase, N-terminal domain Glycolysis, magnesium ion binding,  phosphopyruvate hydratase activity Cytosol
70 Fructokinase 3 (gi|38604456) pfkB family carbohydrate kinase D-ribose metabolic process, ribokinase activity Chloroplast
83 Enolase (gi|119354) Enolase, N-terminal domain Glycolysis, phosphopyruvate hydratase complex, magnesium ion binding;  phosphopyruvate hydratase activity Cytosol
86 Adenosine kinase isoform 1T (gi|51949796) pfkB family carbohydrate kinase AMP biosynthetic process, purine ribonucleoside salvage,  adenosine kinase activity,  phosphotransferase activity, alcohol group as acceptor.\ Chloroplast, Cytosol, Mitochondria
90 LEXYL2 (gi|37359708) Glycosyl hydrolase family 3 Carbohydrate metabolic process, hydrolase activity, hydrolyzing O-glycosyl compounds Cytosol, Mitochondria
92 Hypothetical protein OsI_31140 (gi|125563499) Enolase, N-terminal domain Glycolysis, trichome morphogenesis, magnesium ion binding; phosphopyruvate hydratase activity Cytosol, Nucleus
Amino acid transport and metabolism (E)      
63 Glutamine synthetase GS1 (gi|209529862) Glutamine synthetase, beta-Grasp domain Glutamine biosynthetic process, nitrogen compound metabolic process, ATP binding; glutamate-ammonia ligase activity Chloroplast, Cytosol
71
Cysteine synthase, chloroplastic / chromoplastic (gi|11131628) Pyridoxal-phosphate dependent enzyme Cysteine biosynthetic  process from serine, cysteine synthase activity, transferase activity Chloroplast, Mitochondria
75 Glutamine synthetase GS58 (gi|40457328) Glutamine synthetase, beta-Grasp domain Glutamine biosynthetic process; nitrogen compound metabolic process, ATP binding; glutamate-ammonia ligase activity Chloroplast, Mitochondria
78 Leucine aminopeptidase 2, chloroplastic (gi|2492530) Cytosol aminopeptidase family Proteolysis, aminopeptidase activity, manganese ion binding, metalloexopeptidase activity Chloroplast, Cytosol
DNA Replication, recombination, and repair (L)    
40 Replication factor A 1, rfa1, putative (gi|255546005) Replication factor-A protein 1 DNA replication, DNA binding Cell wall, Chloroplast, Cytosol, Nucleus
Secondary metabolites biosynthesis, transport and catabolism (Q)/ General function prediction only (R)  
41 Cysteine protease Cp1(gi|146215994) Cathepsin propeptide inhibitor domain (I29) Proteolysis, cysteine-type peptidase activity Vacuole
49 Tropinone reductase 1 (gi|1717752) short chain dehydrogenase Tropane alkaloid biosynthetic process, nucleotide binding,  tropine dehydrogenase activity Chloroplast
74 Caffeic acid O-methyltransferase (gi|12003964) Dimerisation domain Lignin biosynthetic process, caffeate O-methyltransferase activity Chloroplast
Lipid metabolism (I)/ Secondary metabolites biosynthesis, transport and catabolism (Q)  
76, 77 3-oxoacyl-[acyl-carrier-protein] synthase (gi|3599489) Beta-ketoacyl synthase, N-terminal domain Fatty acid biosynthetic process, 3-oxoacyl-[acyl-carrier-protein] synthase activity Chloroplast
Cell envelope biogenesis, outer membrane (M)/ Carbohydrate transport and metabolism (G)  
57 Phenylcoumaran benzylic ether reductase (gi|213385143) NmrA-like family Nucleotide binding. Cytosol
No known COG      
16 Transposon protein, putative, CACTA, En/Spm sub-class (gi|77553508) No pfam No related GO Chloroplast
18 P23 protein (gi|587546) Translationally controlled tumour protein No related GO Cytosol
20 Conserved hypothetical protein (gi|255542318) PITH domain Cell redox homeostasis, glycerol ether metabolic process, electron carrier activity; protein disulfide oxidoreductase activity Chloroplast, Cytosol, Nucleus
21 Pathogenesis-related protein 10 (gi|60542787) Pathogenesis-related protein Bet v I family Defense response, response to biotic stimulus Cytosol
23 Putative transcription factor Btf3 (gi|121551087) NAC domain No related GO Nucleus
25, 26 Pathogenesis-related protein 10 (gi|85700977) Pathogenesis-related protein Bet v I family Defense response, response to biotic stimulus Cytosol
103, 104 Abscisic stress ripening protein (gi|607905) ABA/WDS induced protein Response to stress Nucleus
13, 14 Putative pathogenesis related protein (gi|58531054) Pathogenesis-related protein Bet v I family Defense response, response to biotic stimulus Cytosol
5 Predicted protein (gi|168009197) Profilin Actin cytoskeleton organization Chloroplast, Cytosol
32 23kDa polypeptide of O2 evolving complex of photosystem II (gi|146454486) PsbP Photosynthesis, calcium ion binding Chloroplast
34 Protein P21 (gi|129320) Thaumatin family No related GO Vacuole
28 Pathogenesis-related protein R major form (gi|131015) Thaumatin family Defense response, response to biotic stimulus Mitochondria
51 Fibrillin (gi|460761) PAP_fibrillin Structural molecule activity Chloroplast
52 Unknown (gi|255638262) Manganese-stabilising protein / photosystem II polypeptide  Photosynthesis, oxygen evolving complex, calcium ion binding Chloroplast
53 Chloroplast managanese stabilizing protein (gi|283049930) Manganese-stabilising protein / photosystem II polypeptide Photosynthesis,  photosystem II stabilization, oxygen evolving complex, calcium ion binding Chloroplast
55 Harpin binding protein 1 (gi|38679329) PAP_fibrillin Chloroplast structural molecule activity Chloroplast
58 Predicted protein (gi|224063293) GRAM domain No related GO Cell wall, Nucleus
65 Caffeic acid 3-O-methyltransferase (gi|30315948) Dimerisation domain Lignin biosynthetic process, caffeate O-methyltransferase activity Chloroplast
67 Alpha-galactosidase (gi|34765755) Melibiase Carbohydrate metabolic process, cation binding; raffinose alpha-galactosidase activity Cell wall
69 Putative stress related chitinase (gi|62719021) Podoplanin  No related GO Cell wall
80, 82 UTP--glucose-1-phosphate uridylyltransferase (gi|136739) UTP--glucose-1-phosphate uridylyltransferase  UTP:glucose-1-phosphate  uridylyltransferase activity Cytosol
84 Endopolygalacturonase (gi|78482998) Glycosyl hydrolases family 28 Carbohydrate metabolic process, cellular cell wall organization, polygalacturonase activity Cell membrane
88 Actin (gi|158529884) Actin Cytoskeleton, ATP binding. Cytosol
93 RAD23-like (gi|77745475) Ubiquitin family Nucleotide-excision repair, proteasomal ubiquitin-dependent protein catabolic process, damaged DNA binding Nucleus
94 Calreticulin (gi|11131769) Calreticulin family Protein folding, calcium ion binding Endoplasmic reticulum
95 RAD23 protein (gi|5640111) Ubiquitin family Nucleotide-excision repair, proteasomal ubiquitin-dependent protein catabolic process, damaged DNA binding Nucleus
96 Serine carboxypeptidase III (gi|148469859) Serine carboxypeptidase Proteolysis, serine-type carboxypeptidase activity Peroxisome, Vacuole
aCOGs were assigned after COGnitor search and functional role categories were assigned as per the descriptions in COG page at http://www.ncbi.nlm.nih.gov/COG.
bPlantPLoc: Predicting plant protein subcellular location (http://www.csbio.sjtu.edu.cn/cgi-bin/PlantPLoc.cgi).
Table 3: Proteins identified from ‘Bhut Jolokia’ fruit with functional category, Pfam, and gene ontology (Inferred from Electronic Annotation (IEA).