Figure 2: Protein levels of FABP7, GFAP, PEA-15 and vimentin in WT and Fabp7 KO astrocytes were evaluated by Western blotting. Fabp7 KO and WT astrocyte were lysed and 10 micrograms of cell lysates were resolved by 15% SDS-PAGE and probed by specific antibodies. Density of bands was analyzed using Multi Gauge software, and β-actin was used as loading control to normalize the loading level of proteins. (A) PEA-15 and vimentin expression levels were significantly downregulated in Fabp7 KO astrocytes in comparison with their WT counterparts. No significant change was observed in the expression of GFAP between Fabp7 KO and WT astrocytes. (B) Bar graphs Represent analyses of the relative expression of PEA-15, vimentin, and GFAP in Fabp7 KO and WT astrocytes analyzed by Mann-Whitney U test. Data are presented as mean ± SEM from two independent Western blotting experiments with at least three biological replicates per genotype; *P < 0.05.