spot protein name abbreviated gene name accession no. theoreticalpI theoretical Mr distinct peptides sequence coverage (%) MS/MS search score change inFabp7KO astrocyte (fold) P
1 mitochondrial 10-formyltetrahydrofolate dehydrogenase Aldh1l2 Q8K009 5.5- 6 70- 80 1 1 10.65 - 2 0.009
2 vimentin Vim P20152 4.5- 5 40- 50 3 10 34.33 - 1.6 0.015
3 glial fibrillary acidic protein Gfap P03995 5 40- 50 2 10 28.81 + 1.6 0.013
4 actin, aortic smooth muscle Acta2 P62737 5- 5.5 40- 50 1 2 17.37 - 1.3 0.049
5 nuclear receptor corepressor 1 Ncor1 Q60974 6- 6.5 50- 60 1 0 11.36 - 1.4 0.039
6 COP9 signalosome complex subunit 4 Cops4 O88544 4.5- 5 40 - 50 1 4 11.15 + 1.2 0.023
7 melanoma inhibitory activity protein 3 Mia3 Q8BI84 4.5-5 30-40 1 0 10.72 + 1.4 0.017
8 protein BUD31 homolog Bud31 Q6PGH1 4 - 4.5 15 - 20 1 20 10.14 - 1.5 0.038
9 calmodulin Calm1 P62204 4.5 15- 20 1 10 16.58 - 1.4 0.036
10 DnaJ homolog subfamily C member 5 Dnajc5 P60904 4.5 10- 15 1 10 10.33 + 1.1 0.031
11 hippocalcin-like protein 1 Hpcal1 P62748 4.5-5 15 - 20 1 10 11.17 - 1.2 0.048
12 serrate RNA effector molecule homolog Srrt Q99MR6 5 10 -15 1 2 10.76 + 1.3 0.031
13 astrocytic phosphoprotein PEA-15 Pea15 Q62048 5 10 -15 3 12 40.67 - 1.3 0.016
14 ganglioside GM2 activator Gm2a Q60648 5 20- 30 1 5 11.63 + 1.3 0.027
15 ataxin-7-like protein 3 Atxn7l3 A2AWT3 5.5- 6 20-30 1 5 10.63 + 1.6 0.044
16 fatty acid-binding protein, brain Fabp7 P51880 5.5- 6 10 - 15 3 31 38.79 - 2.1 0.003
Table 1: Identification of proteins that are differentially expressed between Fabp7 KO and WT astrocytes by LC-MS/MS analysis. The data of MS analysis was summarized by the Agilent Spectrum Mill MS proteomics workbench against the Swiss-Prot protein database search engine and MASCOT MS/MS Ions Search engine. Five independent astrocyte cultures from either Fabp7 KO or WT mice were examined (two technical replicates per each sample); spot numbers are same as in Figure 1. (+) and (–) show the upregulated and downregulated proteins in Fabp7 KO astrocytes, respectively.